β-Tubulin Gene Based PCR-RFLP Method for Specific Detection ofBabesia bigeminaandTheileria annulataIsolates
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چکیده
منابع مشابه
benzimidazole -resistance in haemonchus contortus : new pcr-rflp method for the detection of point mutation at codon 167 of isotype 1 β-tubulin gene
b ackground: due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new pcr-rflp technique, based on a modified forward primer (ut- hc167 mf-primer), to identify simultaneously the snps at codons 167 and 200 of isotype 1 β-tubu- lin gene of haemonchus contortus . m ethods: there already are several safe and easy methods for identificati...
متن کاملBenzimidazole -Resistance in Haemonchus contortus: New PCR-RFLP Method for the Detection of Point Mutation at Codon 167 of Isotype 1 β-Tubulin Gene
BACKGROUND Due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new PCR-RFLP technique, based on a modified forward primer (UT-HC167 MF-primer), to identify simultaneously the SNPs at codons 167 and 200 of isotype 1 β-tubulin gene of Haemonchus contortus. METHODS There already are several safe and easy methods for identification of ...
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The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry. Despite efforts to control this disease, the high frequency of new epizootic events indicate that the vaccine and antibiotics treatments have limited effectiveness, therefore the preve...
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surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. in this study beta tubulin gene (bt2), was used as the first time in a pcr-rflp format to clarify the distribution of dermatophytosis agents in some parts of iran.a total of 603 clinical isolates was obtained from 500 patients in tehran, isfahan, mazanda...
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ژورنال
عنوان ژورنال: Journal of Applied Animal Research
سال: 2010
ISSN: 0971-2119,0974-1844
DOI: 10.1080/09712119.2010.9707131